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	Provedor de dados Electron. J. Biotechnol. (1) Autor Li,Aiqin (1) Wang,Xingjun (1) Xia,Han (1) Yuan,Mei (1) Zhang,Yue (1) Zhao,Chuanzhi (1) Mais... Palavra-chave Arachis hypogaea L. (1) CDNA library (1) Chalcone isomerase (1) Expression analysis (1) Tipo do documento Journal article (1) Ano 2012 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene Electron. J. Biotechnol. Zhang,Yue; Xia,Han; Yuan,Mei; Zhao,Chuanzhi; Li,Aiqin; Wang,Xingjun. Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than... Tipo: Journal article Palavras-chave: Arachis hypogaea L.; CDNA library; Chalcone isomerase; Expression analysis. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100005 Registros recuperados: 1 Primeira ... 1 ... Última

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